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目的和方法：本实验采用细胞ＥＬＩＳＡ和ＲＴ－ＰＣＲ的方法分别从蛋白质和ｍＲＮＡ水平对血管紧张素Ⅱ（Ａｎｇ－Ⅱ）作用下的内皮细胞表面整合素β３的表达进行检测。结果：１０－８ｍｏｌ／Ｌ～１０－５ｍｏｌ／Ｌ的Ａｎｇ－Ⅱ在蛋白质水平能够促进内皮细胞整合素β３表达（Ｐ＜０．０５），且呈剂量依赖性。１０－６ｍｏｌ／Ｌ的Ａｎｇ－Ⅱ作用培养的人脐静脉内皮细胞１８ｈ后，细胞整合素β３ｍＲＮＡ量为正常对照组的１５倍。结论：提示Ａｎｇ－Ⅱ促进内皮细胞表面整合素β３的表达可能是单核细胞和内皮细胞之间粘附功能增加的机制之一。
PURPOSE AND METHODS: The expression of integrin β3 on the surface of endothelial cells under the action of angiotensin Ⅱ (Ang-Ⅱ) was detected by ELISA and RT-PCR from protein and mRNA levels. Results: Angiotensin Ⅱ levels of 10-8mol / L ~ 10-5mol / L promoted the expression of integrin β3 (P <0.05) at a protein level in a dose-dependent manner. The amount of integrin β3 mRNA in human umbilical vein endothelial cells incubated with 10-6mol / L Ang-Ⅱ for 18h was 15 times of that of the normal control. Conclusion: Ang-Ⅱ may promote the expression of integrin β3 on endothelial cells and may be one of the mechanisms of the increased adhesion between monocytes and endothelial cells.