应用体外细胞培养及流式细胞仪技术，分析了丹参酮作用前后HL－60细胞的细胞周期分布、细胞增殖指数、p53、c－myc、c－fos及bc1－2基因产物的表达变化，并以全反式维甲酸为对照。结果表明：0．5μg．ml－1的丹参酮作用后，HL－60细胞趋向粒细胞系统分化，有部分细胞发生凋亡（11．8％），丹参酮通过阻止HL－60细胞进入S期而抑制其DNA合成，其作用的分子机制与c－fos基因的表达增高、c－myc基因及bc1－2基因的表达降低有关，诱导分化与细胞凋亡的关系尚有待进一步探讨。 Using in vitro cell culture and flow cytometry, the cell cycle distribution, cell proliferation index, expression changes of p53, c-myc, c-fos, and bcl-2 gene products of HL-60 cells before and after tanshinone were analyzed. All-trans retinoic acid served as a control. The results showed: 0.5μg. After the action of ml-1 tanshinone, HL-60 cells differentiated in the granulocyte system, and some cells were apoptosed (11.8%). Tanshinone inhibited DNA synthesis by preventing HL-60 cells from entering S phase. The molecular mechanism is related to the increased expression of c-fos gene and the decreased expression of c-myc gene and bcl-2 gene. The relationship between induced differentiation and apoptosis remains to be further explored.