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为了探索维甲酸用于喉癌保守治疗的可能性，我们研究了它在体外对人喉癌细胞株Ｈｅｐ－２的生长抑制作用及癌基因的变化。结果表明，维甲酸浓度为４～８μｍｏｌ／Ｌ时抑制率可达３５．４％，并使Ｈｅｐ－２细胞的软琼脂克隆形成率降至对照的１９．５％；核酸探针杂交表明，细胞经１０μｍｏｌ／Ｌ维甲酸处理３天后Ｃ－ｍｙｃ平均拷贝数由９．２降至１．２，Ｃ－ｍｙｃ及ｃ－Ｈａ－ｒａｓ的ＲＮＡ水平分别降至对照的１５．３％和５０．５％；ｃ－ｅｒｂＢ－２基因处理后无显著变化。上述结果说明，维甲酸在体外对喉癌细胞株Ｈｅｐ－２的生长有明显抑制作用且与Ｃ－ｍｙｃ及ｃ－Ｈａ－ｒａｓ基因的调控有重要关系。
In order to explore the potential of retinoic acid for the conservative treatment of laryngeal cancer, we studied its in vitro growth inhibitory effect on human laryngeal carcinoma cell line Hep-2 and oncogene changes. The results showed that when the concentration of retinoic acid was 4 ~ 8μmol / L, the inhibition rate was 35.4%, and the soft agar colony formation rate of Hep-2 cells was reduced to 19.5% of the control. The hybridization of nucleic acid probes showed that the cells The average C-myc copy number decreased from 9.2 to 1.2 after 3 days of treatment with 10 μmol / L retinoic acid, and the RNA levels of C-myc and c-Ha-ras decreased to 15.3% and 50.5% of the control, respectively %; No significant change after c-erbB-2 gene treatment. These results indicate that retinoic acid in vitro significantly inhibited the growth of laryngeal carcinoma cell line Hep-2 and is closely related to the regulation of C-myc and c-Ha-ras genes.