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用高效液相－电化学检测器和旋转计数方法检测6－羟多巴胺（6－hydroxydopamine，6－OHDA）损毁大鼠纹状体多巴胺（dopamine，DA)排空与旋转的关系。D1/D2混合性激动剂阿扑吗啡（apomorphine，APO，0．2mg/kg，iP）可使33只大鼠中的20只出现旋转．其中15只旋转强度＞5次／分钟，平均为12．l±5．8次／分钟。D1选择性激动剂SKF38393、D2选择性激动剂LY171555有类似APO的作用。然而，左旋千金藤啶碱（（-）－stepholidine,SPD)仅使14只大鼠出现旋转，其中6只旋转＞5次/分钟，平均为6．41±1．3次/分钟。33只损毁大鼠健测纹状体DA合量平均为9．9±1．1μg/g湿组织，损侧为2．8±2．9μg/g湿组织。旋转＞5次／分钟的大鼠，其健侧和损侧纹状体内DA含量分别为10．1±0．8和0．3±0．1μg/g湿组织。说明纹状体内DA排空程度与旋转行为有密切关系。当损侧纹状体排空小于51％时．APO不能引起任何旋转，而SPD的阈值为88．2％。APO、SKF38393、LY171555和SPD引起＞5次／分钟的旋转，分别需要损侧纹状体内DA排空达96±4％，94±4％，95±4％和98±2％以上。
The relationship between the emptying and the rotation of dopamine (DA) in the striatum of 6-hydroxydopamine (6-OHDA) -deficient rats was detected by high performance liquid chromatography-electrochemical detector and spin counting method. The D1 / D2 mixed agonist apomorphine (APO, 0.2 mg / kg, iP) caused 20 out of 33 rats to rotate. Of these, 15 had a rotation strength> 5 beats / min with an average of 12. ± 5.8 times / minute. D1 selective agonist SKF38393, D2 selective agonist LY171555 has a similar role of APO. However, only 14 rats were rotated by levamisole (SPD), of which 6 were rotated> 5 beats / min with an average of 6.41 ± 1.3 beats / min. The average amount of DA in 33 injured rats was 9.9 ± 1.1μg / g wet tissue, and the damage side was 2.8 ± 2.9μg / g wet tissue. The DA content in the contralateral and striatal striatum of rats that were rotated> 5 beats / min was 10.1 ± 0.8 and 0.3 ± 0.1 μg / g wet tissue, respectively. It shows that there is a close relationship between the degree of DA evacuation in the striatum and the rotation behavior. When the injured side of the striatum less than 51% emptying. APO can not cause any rotation, while the SPD threshold is 88.2%. APO, SKF38393, LY171555 and SPD caused> 5 beats / minute rotation, requiring 96 ± 4%, 94 ± 4%, 95 ± 4% and 98 ± 2% DA depletion in the striatum, respectively.