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目的探讨冠心病伴抑郁障碍与5-羟色胺转运体基因及纤溶酶原激活物抑制物-1基因4G/5G多态性的关系。方法选择对照组63例、单纯抑郁组56例、单纯冠心病组91例和冠心病伴抑郁组75例,采用聚合酶链反应技术检测受试对象5-羟色胺转运体基因上游调控区多态性位点(5-HTTLPR)与内含子2区(5-HTTVNTR)及纤溶酶原激活物抑制物-1(PAI-1)基因4G/5G多态性的基因型。结果单纯抑郁组、冠心病伴抑郁组5-HTTLPR的SS基因型(分别为50%和51%)及S等位基因频率(分别为0.69和0.68)明显高于对照组和单纯冠心病组(P<0.05);单纯抑郁组、冠心病伴抑郁组LL基因型(分别为13%和15%)及L等位基因频率(分别为0.31和0.32)显著低于对照组和单纯冠心病组(P<0.05)。单纯冠心病组、冠心病伴抑郁组PAI-1基因4G/4G基因型(分别为47%和48%)及4G等位基因频率(均为0.68)显著高于对照组和单纯抑郁组(P<0.05);单纯冠心病组、冠心病伴抑郁组5G/5G基因型(均为12%)及5G等位基因频率(均为0.32)显著低于对照组和单纯抑郁组(P<0.05),5-HTTVNTR基因型(12/12,12/10,10/10)分布各组差异无显著性(P>0.05),冠心病伴抑郁组SS+4G/4G联合基因型显著高于对照组、单纯抑郁组和单纯冠心病组(分别为27%、13%、13%和10%,均P<0.05)。结论5-HTTLPR基因SS基因型和PAI-1基因4G/4G基因型共存与冠心病伴抑郁障碍发病相关。
Objective To investigate the relationship between polymorphisms of 4G / 5G and serotonin transporter gene and plasminogen activator inhibitor-1 gene in patients with coronary heart disease and depression. Methods Sixty-three cases of control group, 56 cases of simple depression group, 91 cases of simple coronary heart disease group and 75 cases of coronary heart disease and depression group were selected. Polymerase chain reaction (PCR) was used to detect the polymorphism of upstream regulatory region of serotonin transporter (5-HTTLPR) and intron 2 (5-HTTVNTR) and plasminogen activator inhibitor-1 (PAI-1) gene 4G / 5G polymorphism. Results The SS genotypes (50% and 51%, respectively) and S allele frequencies (0.69 and 0.68, respectively) of 5-HTTLPR group were significantly higher than those of control group and CHD group P <0.05). LL genotypes (13% and 15%, respectively) and L allele frequencies (0.31 and 0.32, respectively) in patients with depression alone, coronary heart disease and depression group were significantly lower than those in control group and CHD group P <0.05). The 4G / 4G genotypes (47% and 48%, respectively) and the 4G allele frequencies (all 0.68) of PAI-1 gene in CHD group and CHD group were significantly higher than those in control group and depression group <0.05). The frequencies of 5G / 5G genotype (both 12%) and 5G allele (all 0.32) in CHD group, CHD group and depression group were significantly lower than those in control group and depression group (P <0.05) (P> 0.05). The combined genotypes of SS + 4G / 4G in patients with coronary heart disease and depression were significantly higher than those in the control group , Simple depression group and simple coronary heart disease group (27%, 13%, 13% and 10%, respectively, P <0.05). Conclusions The coexistence of SS genotype of 5-HTTLPR gene and 4G / 4G genotype of PAI-1 gene is associated with the pathogenesis of CHD with depression.