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痢疾杆菌是小儿细菌性腹泻的主要病原体,被卫生部列为第三类病原微生物。文章为了给小儿痢疾抗药性研究提供基因表达差异性信息,用左氧氟沙星处理诱导痢疾杆菌使其产生抗药性,提取抗药和非抗药痢疾杆菌的总RNA,以随机引物逆转录获得c DNA,对转录本进行建库测序,分析其基因表达差异情况。结果显示痢疾杆菌菌耐药菌株与原始菌株相比有明显的基因差异表达,差异性表达基因占19.84%,其中4.7%基因上调表达,15.1%基因下调表达,且基因表达调控有关的sRNA也呈明显的差异性表达。对两个转录本的基因功能注释表明双组份系统和主动排外泵相关的基因表达有明显差异,这可能与菌株的抗药性产生有关。
Shigella is the main pathogen of bacterial diarrhea in children, the Ministry of Health as the third group of pathogenic microorganisms. In order to provide genetic information for pediatric dysentery drug resistance, we used levofloxacin to induce Shigella dysenteriae to produce drug resistance, and extracted the total RNA of drug-resistant and non-drug-resistant Shigella dysenteriae. Reverse transcription of c DNA with random primers Transcripts were constructed and sequenced, and their differences in gene expression were analyzed. The results showed that the drug-resistant strains of Shigella flexneri were significantly differentially expressed compared with the original strain, accounting for 19.84% of the total, of which 4.7% were up-regulated and 15.1% were down-regulated, and the sRNAs related to gene expression were also Significant difference in expression. Functional annotation of the two transcripts showed significant differences in gene expression between the two-component system and active xenotransplantation, which may be related to the resistance of the strains.