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目的 :研究长期喉返神经损伤后疑核运动神经元睫状神经营养因子 (CNTF)的表达及分布。方法 :以犬单侧喉返神经损伤为实验模型 ,采用原位杂交及免疫组织化学技术 ,结合图像分析技术测定疑核 CNTF m RNA及其蛋白表达呈阳性反应的神经元胞体灰度、体积及数量。 结果 :疑核运动神经元能自分泌 CNTF,神经损伤 3周内神经元 CNTF基因表达强度及反应的胞体数明显下降 ,而 CNTF蛋白表达增强 ,但表达的胞体数无改变。 4周后基因表达强度和反应的胞体数迅速增高 ,而CNTF表达强度恢复正常 ,反应的胞体数无变化。 6周后基因及其蛋白表达和神经元胞体数及体积均始终低于健侧。 12周后CNTF及其蛋白表达、神经元胞体数及体积趋向稳定。结论 :长期喉返神经损伤疑核运动神经元仍保持较高水平的 CNTF表达 ,从中枢及神经营养因子角度提示晚期周围神经损伤具有修复价值
Objective: To study the expression and distribution of ciliary neurotrophic factor (CNTF) in the nucleus accumbens after long-term recurrent laryngeal nerve injury. Methods: The unilateral recurrent laryngeal nerve injury in canine was used as an experimental model. The in situ hybridization and immunohistochemistry were used to detect the gray value and volume of neuronal cell bodies with suspected nuclear CNTF m RNA and protein expression. Quantity. Results: CNTF neurons were capable of autocrine CNTF. The intensity of CNTF gene expression and the number of somatic cells were significantly decreased in 3 weeks after nerve injury, while the expression of CNTF protein was enhanced but the number of somatic cells did not change. After 4 weeks, the intensity of the gene expression and the number of the somatic cells rapidly increased, while the expression intensity of CNTF returned to normal, with no change in the number of somatic cells. After 6 weeks, the gene and its protein expression and neuronal cell number and volume were always lower than the contralateral side. After 12 weeks, the expression of CNTF and its protein, neuronal cell number and volume tended to be stable. CONCLUSION: Long-term recurrent laryngeal nerve injury still possesses high level of CNTF expression in suspected motoneurons. It suggests that the repair of advanced peripheral nerve injury from the perspective of central and neurotrophic factors