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目的观察酪氨酸激酶在多株黑素瘤细胞系及原代表皮黑素细胞中的表达,探讨酪氨酸激酶在黑素瘤发生发展过程中的作用。方法分离并培养人原代表皮黑素细胞,培养浅表垂直生长期黑素瘤细胞株WM793B,转移性黑素瘤细胞株A2058,WM-266-4,Hs294T,SK-MEL-5和SK-MEL-1,用RT-PCR法分别检测7株细胞中酪氨酸激酶c-abl,c-kit和PDGFRα/βmRNA的表达;Western-blot法检测7组细胞中非受体性酪氨酸激酶c-abl蛋白水平的表达。结果与表皮黑素细胞和WM793B细胞相比,5株转移性黑素瘤细胞中的c-ablmRNA及其蛋白水平的表达偏高;而PDGFRα/βmRNA表达明显偏低、c-kitmR-NA在表皮黑素细胞,A2058,WM-266-4和Hs294T中mRNA表达较高,而在WM793B,SK-MEL-5和SK-MEL-1中偏弱。结论酪氨酸激酶c-abl对黑素瘤的发生发展可能有促进作用,而PDGFRα/βmRNA的低表达可能与黑素瘤的转移有关。
Objective To observe the expression of tyrosine kinases in multiple melanoma cell lines and primary epidermal melanocytes and to explore the role of tyrosine kinases in the development of melanoma. Methods Human primary epidermal melanocytes were isolated and cultured. The superficial vertical growth phase melanoma cell line WM793B, metastatic melanoma cell lines A2058, WM-266-4, Hs294T, SK-MEL-5 and SK- The expression of tyrosine kinase c-abl, c-kit and PDGFRα / βmRNA in 7 cells were detected by RT-PCR and MEL-1. The expression of non-receptor tyrosine kinase c-abl protein level. Results Compared with epidermal melanocytes and WM793B cells, the expression of c-abl mRNA and its protein in 5 metastatic melanoma cells was high, while the expression of PDGFRα / β mRNA was significantly lower. The expression of c-kitmR-NA in epidermis The mRNA expression of melanocytes, A2058, WM-266-4 and Hs294T was higher, but weakened in WM793B, SK-MEL-5 and SK-MEL-1. Conclusion The tyrosine kinase c-abl may promote the development of melanoma, while the low expression of PDGFRα / βmRNA may be related to the metastasis of melanoma.