论文部分内容阅读
目的 观察大鼠海马内生长抑素在癫中的作用。方法 14肽生长抑素(SS14)或半胱胺微量注入背侧海马,在大鼠感觉运动皮质记录脑电功率,应用推挽灌流结合放免法测定青霉素致后背侧海马内生长抑素免疫反应物质(SLI)含量的变化。结果 致后0.5hSLI含量显著增加,可持续4h。SLI含量的上升程度与致后感觉运动区脑电相对功率增加呈正相关。单侧背海马内注入SS140.03nmol使大鼠感觉运动区出现棘尖波,行为上表现样活动。单侧背海马内注入SS耗竭剂半胱胺30μg减少SLI含量30%左右,原先致剂量青霉素所诱发的癫程度减轻。结论 背侧海马SS可能参与致
Objective To observe the role of somatostatin in epilepsy in hippocampus of rats. METHODS 14 Peptide somatostatin (SS14) or cysteamine was injected into the dorsal hippocampus by microinjection into the dorsal hippocampus. EEG power was recorded in the sensory motor cortex in rats. The somatostatin immunoreactivity in the dorsal hippocampus was determined by push-pull perfusion and radioimmunoassay Changes in substance (SLI) content. RESULTS: After 0.5 h, the content of SLI significantly increased for 4 h. There was a positive correlation between the increase of SLI level and the relative power of EEG in sensory zone. SS140.03nmol was infused into the unilateral dorsal hippocampus to induce spike waves in the sensory area of the rats. Single-sided dorsal hippocampus injection of SS depletion agent cysteamine 30μg to reduce the SLI content of about 30%, originally caused by the dose of penicillin induced epilepsy reduced. Conclusion The dorsal hippocampus SS may be involved in the carcinogenesis