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太谷核不育小麦的育性受单个显性雄性不育基因(Ta1)控制,其不育株总是杂合(Ta1ta1)的,纯合不育株(Ta1Ta1)并不存在。实验以太谷核不育小麦(TriticumaestivumL.)为母本和玉米(ZeamaysL.)杂交,利用杂合子和幼胚细胞分裂过程中父本玉米染色体自发消除的特点,经过激素处理、幼胚拯救和染色体人工加倍,成功地获得了自然界不存在的纯合显性太谷核不育小麦新种质(Ta1Ta1),并利用“玻璃化”超低温保存方法,将这一宝贵新种质长期保存下来。
The fertility of Taigu nuclear sterile wheat was controlled by a single dominant male sterile gene (Ta1). The sterile lines were always heterozygous (Ta1ta1), and the homozygous sterile lines (Ta1Ta1) were absent. In this study, Triticum aestivum L. was crossed with maize (Zea mays L.) using the characteristics of spontaneous elimination of maternal chromosomes during the process of heterozygous and immature embryo cell division. After hormone treatment, embryo rescue and chromosome Artificial doubling, successfully obtained in nature does not exist homozygous dominant Taigu nuclear sterile wheat germplasm (Ta1Ta1), and the use of “vitrification” cryopreservation save this precious new germplasm long-term preservation.