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目的:建立广东土牛膝药材的指纹图谱。方法:采用高效液相色谱法建立10批广东土牛膝药材的指纹图谱,用液相色谱-质谱联用技术标记指纹图谱中的主要色谱峰。结果:获得的指纹图谱有16个共有峰,聚类分析结果将10批药材分为两类,根据质谱提供的信息标记了5个共有峰。结论:广东土牛膝药材指纹图谱的特征性及专属性强,可用于药材的质量控制。
Objective: To establish the fingerprints of Achyranthes bidentata in Guangdong Province. Methods: Fingerprints of 10 batches of Achyranthes bidentata L. were prepared by high performance liquid chromatography (HPLC), and the main chromatographic peaks of fingerprints were identified by liquid chromatography-mass spectrometry. Results: There were 16 common peaks in the obtained fingerprints. The results of cluster analysis divided the 10 batches of herbs into two categories and labeled five common peaks according to the information provided by mass spectrometry. Conclusion: The fingerprints of Achyranthes bidentata herbs are characteristic and specific, and can be used for the quality control of medicinal materials.