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小鼠脾细胞经两次贴壁除去巨噬细胞,再经两次尼龙毛柱纯化T细胞,尼龙毛粘附的B细胞用抗θ血清加补体去除残余的T细胞后,得到高度纯化的B细胞,在体外用细胞重组实验方法观察B细胞在ConA反应中的作用。结果表明,静止B细胞和细菌脂多糖活化的B细胞均能非常显著地辅佐T细胞对ConA刺激的增殖反应,B细胞这种作用不受组织相容性抗原约束。B细胞还能辅佐T细胞产生IL-2,这证明B细胞具有辅佐细胞功能。
Mice spleen cells were removed by two adherent macrophages, and then by two nylon column purification of T cells, nylon wool adhesion of B cells with anti-serum plus complement removal of T cells to obtain highly purified B Cells, in vitro experiments using cell recombination observed B cells in ConA response. The results showed that both resting B cells and bacterial lipopolysaccharide-activated B cells can very significantly help T cell proliferation response to ConA stimulation, B cell effect is not bound by histocompatibility antigens. B cells also help T cells to produce IL-2, which proves that B cells have accessory cell functions.