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带有１ ～２ ｍ ｍ 子叶柄的芥菜型油菜子叶经农杆菌感染后，培养在附加１０ ～２０ ｍｇ／ Ｌ卡那霉素的 Ｍ Ｓ 选择培养基上筛选转化愈伤组织及不定芽。卡那霉素抗性苗相继在含３０ ～５０ ｍ ｇ／ Ｌ 卡那霉素的选择培养基上继代培养，再转移到含２０ ｍｇ／ Ｌ 卡那霉素的生根培养基上诱导生根。以苏云金杆菌杀虫晶体蛋白基因为探针，进行 Ｓｏｕｔｈｅｒｎ ｂｌｏｔ 分子杂交，得到阳性结果。 Ｐ Ｃ Ｒ 分析也证明外源基因整合到油菜基因组并稳定传递到后代。转基因植株的抗虫性和卡那霉素抗性在自交后代中得到保持，筛选得到纯合的转基因植株后代株系
After being infected with Agrobacterium tumefaciens with 1 ~ 2 m m petiole, the transformed callus and adventitious buds were screened on M selection medium supplemented with 10 ~ 20 mg / L kanamycin. Kanamycin-resistant seedlings were subcultured on selective medium containing 30-50 kg / L kanamycin and then transferred to rooting medium containing 20 mg / L kanamycin to induce rooting. The Bacillus thuringiensis insecticidal crystal protein gene as a probe, Southern blot hybridization, positive results were obtained. P C R analysis also demonstrated that exogenous genes are integrated into the genome of the canola and are stably passed on to offspring. Insect resistance and kanamycin resistance of the transgenic plants were maintained in the selfing offspring and the progeny lines of the homozygous transgenic plants were screened out