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在广泛搜集、耐热性筛选与鉴定的基础上,从大量的品种资源中选择对灌浆期高温胁迫耐性很强的早籼纯系品种“BG33-2”作供体亲本,灌浆期耐热性敏感的早籼纯系品种“赣早籼38号”为轮回亲本,经过9年11个世代的杂交、连续单株鉴定选择与回交、花药培养、连续自交,育成了一套赣早籼38号近等基因系。33个特征特性的鉴定及性状测定值的统计分析结果表明,本研究育成的G38HT、G38MT、G38HS其性状均已纯合稳定,灌浆期耐热性分别为高抗、中抗、敏感,这3个纯系是灌浆期耐热性有显著差异的赣早籼38号近等基因系。这套近等基因系可为进一步深入系统地探明水稻灌浆期耐热性的生理机制和遗传机理,提供理想的实验材料。
Based on extensive collection and heat resistance screening and identification, the pure line of early indica rice “BG33-2” with high tolerance to high temperature during grain filling stage was chosen as the donor parent from a large number of varieties, The heat-sensitive early indica line “Ganzaoshixian 38” is a reincarnated parental line. After 9 years of 11 generations of crossbreeding, continuous single plant identification and selection, backcrossing, anther culture and continuous selfing have been bred Set Ganzaoxian 38 near isogenic lines. The 33 characteristic traits and the statistical analysis of traits measured showed that the traits of G38HT, G38MT and G38HS bred in this study were homozygous and stable, and the heat tolerance at grain filling stage was highly resistant, moderately resistant and sensitive, respectively A pure line is a near-isogenic line of Ganzaoxian 38 at significant temperature difference during grain filling. This near-isogenic line can provide ideal experimental material for further and systematically ascertaining the physiological mechanism and genetic mechanism of rice’s heat tolerance at grain-filling stage.