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本研究经小麦幼穗细胞和DON毒素混合处理，研究抗赤霉病程度不同的苏麦3号（R）、望水白（R）、延冈坊主（R）、Alondra’s（S）、安徽11（S）和仪宁小麦（S）的可溶性蛋白谱带的变化。用3μg／ml的脱氧雪腐镰刀菌烯酸（DON）与小麦幼穗细胞混和处理2、8、24、30h，分别提取可溶性蛋白，进行PAGE测定。结果显示，感病品种有多条谱带消失，如仪宁小麦、安徽11、Alondra’s在处理2h后Rf0．13的条带消失，处理8h后Rf0．02条带消失，感病品种Alondra’s和安徽11在处理24h后Rf0．05的条带消失。抗病品种的电泳谱带没有变化，如苏麦3号、望水白在毒素处理后蛋白条带没有发生变化，抗病品种延冈坊主在处理24h后RfO．OS、O．11的蛋白带仅有变浅的趋势。研究还表明抗病品种苏麦3号、望水白、延冈坊主具有感病品种没有的Rf0．3的蛋白带，且在毒素处理前后保持一致。
In this study, we studied the effects of sumac 3 (R), Wangshui White (R), Nangang Square (R), Alondra’s (S), Anhui 11 (S) and Yee Ning wheat (S) soluble protein bands changes. With 3μg / ml deoxynivalenol (DON) and wheat young spike cells were mixed for 2,8,24,30h, were extracted soluble protein, PAGE determination. The results showed that there were many bands in susceptible varieties disappeared. For example, the bands of Rf0.13 disappeared after treatment with Alondra’s for 2 h in Yi-Ning wheat, Anhui 11 and disappeared after 8-h treatment. The susceptible varieties Alondra’s and Anhui 11 The band of Rf 0.05 disappeared after 24h treatment. There was no change in the electrophoretic bands of resistant cultivars. For example, Sumai No.3, Wangshuibai did not change the protein bands after toxin treatment, and the resistant cultivars of Nostoc flagelliforme were treated with RfO for 24h. OS, O. 11 protein bands only tend to be lighter. The study also showed that the disease-resistant cultivar Sumai 3, Wangshui Bai and Nangangfang had the protein bands of Rf 0.3 not present in the susceptible cultivars, and remained consistent before and after the toxin treatment.