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为了明确胃癌的血液或淋巴转移和微卫星ＤＮＡ不稳定性（Ｍｉｃｒｏｓａｔｅｌｉｔｅｉｎｓｔａｂｉｌｉｔｙ，ＭＳＩ）及杂合性缺失（Ｌｏｓｓｏｆｈｅｔｅｒｏｚｙｇｏｓｉｔｙ，ＬＯＨ）的关系，选择２９个多态微卫星ＤＮＡ标记（ＭＳ），采用ＰＣＲ－聚丙烯酰胺凝胶电泳及银染技术，分析了３８例胃癌的ＭＳＩ和ＬＯＨ频率。其中胃癌伴有血液或淋巴转移的２４例；不伴有血液或淋巴转移的１４例，结果表明：总的ＭＳＩ和ＬＯＨ频率分别为２０．８４％和２７．５９％，伴有血液或淋巴转移的胃癌，ＭＳＩ在Ｄ７Ｓ５２０、Ｄ８Ｓ２７９位点频率最高为５４．１７％；ＬＯＨ在Ｄ６Ｓ４３０位点频率最高为６２．５０％。无血液或淋巴转移的胃癌，ＭＳＩ和ＬＯＨ频率均为５７．１％。经χ２检验，ＬＯＨ和ＭＳＩ的发生频率在伴有或不伴有血液或淋巴转移胃癌中，两者无统计学差异。表明胃癌的血液或淋巴转移和ＭＳＩ及ＬＯＨ的发生频率无关，目前还难以以这两个指标作为辅助性的判断预后。
In order to clarify the relationship between blood or lymph metastasis of gastric cancer, microsatellite DNA instability (MSI) and loss of heterozygosity (LOH), 29 polymorphic microsatellite DNA markers (MS) were selected and PCR-poly was used. Acrylamide gel electrophoresis and silver staining techniques were used to analyze the frequency of MSI and LOH in 38 cases of gastric cancer. Among them, 24 cases with gastric cancer and blood or lymphatic metastasis; 14 cases without blood or lymphatic metastasis. The results showed that the total frequency of MSI and LOH was 20.84% and 27.59%, respectively, with blood or lymphatic metastasis. In gastric cancer, MSI had the highest frequency of 54.17% in D7S520 and D8S279 loci, and the highest frequency of LOH in D6S430 loci was 62.50%. In gastric cancer without blood or lymphatic metastasis, the frequency of MSI and LOH was 57.1%. After the χ2 test, the frequency of LOH and MSI in gastric cancer with or without blood or lymph node metastasis was not statistically different. It is indicated that the blood or lymph metastasis of gastric cancer is not related to the frequency of MSI and LOH. It is still difficult to use these two indexes as auxiliary prognosis.