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AIM:To investigate free-radical scavenger effect of n-acetylcysteine in rats intragastrically fed with ethanol.METHODS:Twenty-four rats divided into three groups werefed with ethanol (6 g/kg/day,Group 1),ethanol and n-acetylcysteine (1 g/kg,Group 2),or isocaloric dextrose(control group,Group 3) for 4 weeks.Then animals weresacrificed under ether anesthesia,and intracardiac bloodand liver tissues were obtained.Measurements were madein both serum and homogenized liver tissues.Malondialdehyde (MDA) level was measured by TBARSmethod.Glutathione peroxidase (GSH-Px) and superoxidedismutase (SOD) levels were studied by commercial kits.Kruskal-Wallis test was used for statistical analysis.RESULTS:ALT and AST in Group 1 (154 U/L and 302 U/L,respectively) were higher than those in Group 2 (94 U/Land 155 U/L) and Group 3 (99 U/L and 168 U/L) (P=0.001for both).Serum and tissue levels of MDA in Group 1 (1.84nmol/mL and 96 nmol/100 mg-protein) were higher thanthat in Group 2 (0.91 nmol/mL and 64 nmol/100 mg protein)and Group 3 (0.94 nmol/ml and 49 nmol/100 mg-protein)(P<0.001 for both).On the other hand,serum GSH-Px levelin Group 1 (8.21 U/g Hb) was lower than that in Group 2(16 U/g Hb) and Group 3 (16 U/g-Hb) (P<0.001).Serumand liver tissue levels of SOD in Group 1 (11 U/mL and 26U/100 rag-protein) were lower than that in Group 2 (18 U/mL and 60 U/100 mg protein) and Group 3 (20 U/mL and60 U/100 rag-protein) (P<0.001 for both).CONCLUSION:Ethanol-induced liver damage wasassociated with oxidative stress,and co-administration ofn-acetylolsteine attenuates this damage effectively in ratmodel.
AIM: To investigate free-radical scavenger effect of n-acetylcysteine in rats intragastrically fed with ethanol. METHODS: Twenty-four rats divided into three groups were fed with ethanol (6 g / kg / day, Group 1), ethanol and n-acetylcysteine (1 g / kg, Group 2), or isocaloric dextrose (control group, Group 3) for 4 weeks. Animals weresacrificed under ether anesthesia, and intracardiac blood and liver tissues were obtained. Measurements made made both serum and homogenized liver tissues. Malondialdehyde (MDA) level was measured by TBARSmethod.Glutathione peroxidase (GSH-Px) and superoxidedismutase (SOD) levels were studied by commercial kits. Kruskal-Wallis test was used for statistical analysis .RESULTS: ALT and AST in Group 1 (154 U / L and 302 U / L, respectively) were higher than those in Group 2 (94 U / Land 155 U / L) and Group 3 (99 U / L and 168 U / L) tissue levels of MDA in Group 1 (1.84 nmol / mL and 96 nmol / 100 mg-protein) were higher thanthat in Group 2 (0.91 nmol / mL and 64 nmo (p <0.001 for both) .On the other hand, serum GSH-Px levelin Group 1 (8.21 U / g Hb) and Group 3 (0.94 nmol / ml and 49 nmol / Serumand liver tissue levels of SOD in Group 1 (11 U / mL and 26 U / 100 rag) were lower than those in Group 2 (16 U / g Hb) and Group 3 -protein) were lower than that in Group 2 (18 U / mL and 60 U / 100 mg protein) and Group 3 (20 U / mL and 60 U / 100 rag-protein) -induced liver damage wasassociated with oxidative stress, and co-administration of n-acetylolsteine attenuates this damage effectively in rat model.