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目的:通过检测正常妊娠及重度子痫前期患者胎盘组织中miR-19a的表达,探讨其与子痫前期发病的关系。方法:收集10例重度子痫前期患者胎盘组织(实验组)和10例正常产妇胎盘组织(对照组),应用荧光实时定量PCR(Real Time PCR)的方法检测两组miR-19a的表达差异。结果:重度子痫前期患者胎盘组织中miR-19a表达升高(P<0.05)。结论:子痫前期患者胎盘组织中存在差异表达的miRNA,miR-19a在胎盘组织中的高表达可能与子痫前期的发病有关。
OBJECTIVE: To detect the expression of miR-19a in placenta of normal pregnancy and severe preeclampsia and to explore its relationship with the pathogenesis of preeclampsia. Methods: Placenta tissue of 10 patients with severe preeclampsia (experimental group) and 10 normal pregnant women placenta tissues (control group) were collected. Fluorescence real-time PCR was used to detect the difference of miR-19a expression between the two groups. Results: The expression of miR-19a in placenta of patients with severe preeclampsia increased (P <0.05). Conclusion: There are differentially expressed miRNAs in placenta of preeclampsia. The high expression of miR-19a in placenta may be related to the pathogenesis of preeclampsia.