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目的探讨嗜肺军团菌感染对机体血清白细胞介素(IL)12水平及免疫功能的影响。方法 BALB/C小鼠,实验组(又分1、2、3、4周组,n=15,各周第3天时间点观察),腹腔内注射含有嗜肺军团菌菌液0.2ml(1×106/ml),对照组(n=15),腹腔内注射无菌生理盐水0.2ml。分别在感染第1～4周内的相应时间点,采集小鼠血清,用酶联免疫吸附法(ELISA)观察血清IL-12水平的动态变化。结果感染嗜肺军团菌后第1、2、3和4周内的第3天BALB/C小鼠血清IL-12水平分别为(16.16±3.18)、(15.48±3.22)、(16.96±3.43)和(18.12±3.41)μg/ml;对照组相应时间点血清IL-12水平分别为(19.01±3.49)、(19.17±3.95)、(19.58±3.64)和(18.86±3.32)μg/ml;实验组与对照组比,第1～3周差异有统计学意义(P<0.05),而第4周差异无统计学意义(P>0.05);嗜肺军团菌感染后前2周血清IL-12水平下降,第2周达到最低,第3周有上升趋势,第4周基本恢复到正常水平。结论嗜肺军团菌感染后对机体免疫功能早期下降,后期逐渐恢复。
Objective To investigate the influence of Legionella pneumophila infection on serum interleukin (IL) 12 level and immune function. Methods BALB / C mice were randomly divided into experimental group (1, 2, 3, 4 weeks group, n = 15, observed on the 3rd day of each week), intraperitoneal injection of 0.2ml × 106 / ml), control group (n = 15), intraperitoneal injection of sterile saline 0.2ml. Serum was collected from the first to fourth week of infection and the serum level of IL-12 was detected by ELISA. Results The levels of IL-12 in BALB / C mice on the third day after infection with Legionella pneumophila were (16.16 ± 3.18), (15.48 ± 3.22), (16.96 ± 3.43) And (18.12 ± 3.41) μg / ml respectively. The levels of IL-12 in the control group at the corresponding time point were (19.01 ± 3.49), (19.17 ± 3.95), (19.58 ± 3.64) and (18.86 ± 3.32) μg / The difference between the 1st and 3rd week was statistically significant (P <0.05), but there was no significant difference in the 4th week (P> 0.05). The level of IL-12 in the first 2 weeks after Legionella pneumophila infection The lowest level in the second week, the upward trend in the third week and the return to the normal level in the fourth week. Conclusion Legionella pneumophila infection in early immune function decreased, the late recovery.