原代培养人胚肝细胞经１．１５６×１０￣(－７）ｍｏｌ／Ｌ硒预处理４ｈ，加入２０ｍｍｏｌ／Ｌ四氟化碳作用２０ｈ，观察硒对其Ⅲ型前胶原（ＰＣⅢ）和羟脯氨酸（Ｈｙｐ）生成的影响。结果培养液中ＰＣⅢ水平、细胞内Ｈｙｐ含量及细胞内外丙二醛（ＭＤＡ）水平均降低，与未加硒对照组比较差别有显著性（Ｐ＜０．０１）。而硒谷腕甘肽过氧化物酶（Ｓｅ－ＧＳＨ－ＰＸ）活性则较对照组显著增高（Ｐ＜０．００１），且ＰＣⅢ水平与Ｓｅ－ＧＳＨ－Ｐ＿Ｘ／ＭＤＡ比值呈负相关（ｒ＝－０．９１５６，Ｐ＜０．０１）。提示硒可提高Ｓｅ－ＧＳＨ－Ｐ＿Ｘ／ＭＤＡ比值，抑制脂质过氧化激发的肝细胞胶原合成。 Preincubation of human embryonic hepatocytes with selenium (1.156 × 10 ~ (-7) mol / L) for 4 h followed by 20 mmol / L carbon tetrachloride for 20 h observed the effect of selenium on the expression of PC Ⅲ, Effects of proline (Hyp) production. Results The levels of PCⅢ, intracellular Hyp, MDA and MDA in the culture medium were significantly lower than those in the control group without selenium (P <0.01). Se-GSH-PX activity was significantly higher than that of the control group (P <0.001), and the level of PCⅢ was negatively correlated with the Se-GSH-Px / MDA ratio (r = -0.9156, P <0.01). These results suggest that selenium can increase Se-GSH-P_X / MDA ratio and inhibit collagen synthesis induced by lipid peroxidation in hepatocytes.