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溶菌酶是呼吸道防御机制中的重要因素,但它对人鼻粘膜纤毛活动的作用很少报道。作者为弄清楚溶菌酶对纤毛活动的生物学特性,用光电方法在试管内恒温条件下测量了纤毛的活动。本文实验所用为盐酸鸡蛋白溶菌酶。在患慢性鼻窦炎病人鼻窦手术时,取下筛窦粘膜,置无菌冷冻生理盐水中保存。光镜观察确认上皮细胞层和纤毛完好。取出的粘膜标本24小时内进行实验观察。用含0.15MNaCl的0.01M磷酸钠缓冲液(pH7.4)洗去粘膜表面的粘液,然后把标本放在带有喷水装置的室内,与多孔渗透膜一起铺开,室内温度恒定在25℃,纤毛活动至少于3小时内不会改变。用相差显微镜、亚硫酸镉光接受器、示波器和记录器等光电测量装置测量纤毛的运动。在缓冲液怍对
Lysozyme is an important factor in respiratory defense mechanisms, but its effect on human nasal mucociliary activity is seldom reported. To clarify the biological properties of lysozyme on ciliary activity, the authors measured the ciliary activity using a photoelectron method in a test tube at constant temperature. This experiment is used for the egg white lysozyme. In patients with chronic sinusitis sinus surgery, remove the ethmoid mucosa, stored in sterile frozen saline. Light microscopy confirmed epithelial cell layer and cilia intact. Remove the mucosal specimens within 24 hours of experimental observation. The mucus on the mucosal surface was washed away with 0.01 M sodium phosphate buffer (pH 7.4) containing 0.15 M NaCl, and the sample was placed in a room with a water jet apparatus and spread with the porous membrane, keeping the temperature constant at 25 ° C , Cilia activity does not change within at least 3 hours. Ciliary movement was measured with a phase-contrast microscope, a cadmium sulfite photoreceptor, an oscilloscope and a recorder. Right in the buffer