论文部分内容阅读
目的:通过检测体能、学习记忆能力、抗氧化和脂质过氧化等指标,研究紫苏油对D-半乳糖亚急性衰老模型大鼠的抗衰老作用,初步探讨紫苏油抗衰老的可能机制。方法:将40只4周龄SD大鼠按照随机数字表法随机分为5组:阴性对照组、衰老模型组、紫苏油小剂量组(0.815mL/kg)、紫苏油中剂量组(2.445mL/kg)和紫苏油大剂量组(7.337mL/kg),每组8只。除阴性对照组注射生理盐水外,其余各组大鼠均在颈部皮下注射D-半乳糖(125mg/kg)。喂养8周后,进行负重游泳实验和Morris水迷宫实验,并测定血清中超氧化物歧化酶(SOD)和丙二醛(MDA)和脑组织中一氧化氮合酶(NOS)和一氧化氮(NO)。结果:与模型组相比,紫苏油各剂量组大鼠负重游泳时间明显增加(P<0.05);与模型组相比,紫苏油各剂量组大鼠的逃避潜伏期也明显少于模型组(P<0.05),紫苏油小剂量组的有效时间比高于模型组(P<0.05),紫苏油小剂量组的穿越平台次数无明显差异,紫苏油中剂量组和紫苏油大剂量组有效时间比和穿越平台次数均明显高于模型组(P<0.05);与模型组相比,紫苏油所有剂量组的SOD活力明显高于模型组(P<0.05),MDA含量明显低于模型组。结论:成功复制D-半乳糖亚急性衰老大鼠模型;紫苏油对衰老大鼠的体能和学习记忆能力有促进作用,并提高机体SOD活力,降低MDA含量,提示紫苏油确有抗衰老功效;紫苏油的抗衰老功效可能是通过减少血液中的自由基来发挥作用。
OBJECTIVE: To study the anti-aging effects of perilla oil on rat model of D-galactose subacute aging by examining physical fitness, learning and memory abilities, antioxidation and lipid peroxidation, and to explore the possible mechanism of anti-aging of perilla oil . Methods: 40 4-week-old SD rats were randomly divided into 5 groups according to random number table: negative control group, aging model group, low-dose perilla oil group (0.815mL / kg), perilla oil medium dose group 2.445mL / kg) and perilla oil high-dose group (7.337mL / kg), 8 in each group. Except for the negative control group, physiological saline was injected into the other groups, and all rats in the other groups were injected subcutaneously with D-galactose (125 mg / kg) in the neck. After 8 weeks of feeding, the rats were subjected to a load-bearing swimming test and a Morris water maze test. The levels of serum superoxide dismutase (SOD), malondialdehyde (MDA) and brain tissue nitric oxide synthase (NOS) and nitric oxide NO). Results: Compared with the model group, the weight-bearing swimming time was significantly increased in each dose of perilla oil (P <0.05). Compared with the model group, the escape latency of each dose of perilla oil group was also significantly less than that of the model group (P <0.05). The effective time of perilla oil low dose group was higher than that of model group (P <0.05), and the perilla platelet number of perilla oil low dose group had no significant difference. Perilla oil medium dose group and perilla oil (P <0.05). Compared with the model group, the SOD activity of all the perilla oil groups was significantly higher than that of the model group (P <0.05), while the content of MDA Obviously lower than the model group. Conclusion: The rat model of sub-acute aging of D-galactose was successfully replicated. Perilla oil promoted the physical and learning and memory abilities of aged rats, increased SOD activity and decreased MDA content, suggesting that perilla oil does have anti-aging effect Efficacy; perilla oil anti-aging effect may be through the reduction of free radicals in the blood to play a role.