论文部分内容阅读
目的探讨mdx小鼠不同时期骨骼肌的炎性病理改变,观察mpeg1在mdx小鼠及对照鼠中的表达。方法选取雄性C57BL/10ScSn-Dmdmdx/JNju鼠为实验组,对照组为雄性C57BL/6Sc Sn小鼠,根据年龄分为2 w、4 w、8 w、12 w 4个亚组。通过HE染色、MGT染色、ACP染色观察骨骼肌光镜下的形态学改变,总结mdx小鼠骨骼肌炎性病理变化。通过RNA提取,基因芯片的检测及mpegl的qRT-PCR检测,观察mpeg1的表达情况。结果常规组织染色中,2 w的mdx小鼠肌肉偶见高度浓染的肌纤维,未见肌细胞坏死,炎症细胞浸润,4 w可见巨噬细胞吞噬现象散在分布,8 w时炎细胞浸润灶融合成片,12 w时炎性病灶面积减小;利用基因芯片技术筛选出mdx小鼠中有关炎症反应的基因30余个,结果显示与2 w相比,炎症反应相关基因表达量均增加,在8 w时达到峰值,12 w有所下降,但较2 w时仍有升高;qRT-PCR结果显示从4 w开始,mdx小鼠中mpeg1的含量逐渐增加,8 w时含量最高。结论 (1)炎症反应参与mdx小鼠疾病的发生发展:从2 w开始出现,8 w达到高峰,12 w趋于平稳;(2)Mpeg1在mdx小鼠炎症反应中发挥了一定的作用。
Objective To investigate the inflammatory pathological changes of skeletal muscle in mdx mice at different stages and to observe the expression of mpeg1 in mdx mice and control mice. Methods Male C57BL / 10ScSn-Dmdmdx / JNju mice were selected as the experimental group and male C57BL / 6Sc Sn mice as control group, which were divided into 4 subgroups at 2 w, 4 w, 8 w and 12 w according to their ages. Morphological changes under skeletal muscle light microscope were observed by HE staining, MGT staining and ACP staining, and inflammatory pathological changes of skeletal muscle of mdx mice were summarized. The expression of mpeg1 was detected by RNA extraction, microarray detection and mpegl qRT-PCR. Results In normal tissue staining, muscle fibers of 2-day-old mdx mice occasionally showed highly concentrated myofibers. No muscle cell necrosis and infiltration of inflammatory cells were observed. Phagocytosis of macrophages was observed after 4 weeks. Inflammatory cell infiltration was found at 8 weeks And the area of inflammatory lesions decreased at 12 weeks. More than 30 genes related to inflammatory reaction in mdx mice were screened by gene chip technology. The results showed that compared with 2 weeks, the expression of inflammatory response related genes increased, Peaked at 8 w, decreased at 12 w, but still increased at 2 w. The result of qRT-PCR showed that mpeg1 increased gradually in mdx mice from 4 w and reached the highest level at 8 w. Conclusions (1) Inflammatory responses are involved in the development of the disease in mice with mdx onset. The onset of the disease was observed at 2 w, peaked at 8 w and stabilized at 12 w. (2) Mpeg1 played a role in the inflammation of mdx mice.