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目的:研究中国南海简易扁板海绵Plakortis simplex的化学成分。方法:运用正相硅胶柱色谱、反相硅胶柱色谱(ODS)、凝胶柱色谱(Sephadex LH-20)以及高效液相色谱(HPLC)等多种手段对中国南海简易扁板海绵P.simplex的化学成分进行分离纯化;通过理化性质、波谱分析方法并结合文献对照,鉴定化合物的结构。结果:从该海绵的甲醇提取物中共分离鉴定了7个化合物:(22E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol(1),3-吲哚甲酸(2),3-吲哚甲醛(3),monotriajaponide A(4),methyl(2Z,6R,8R,9E)-3,6-epoxy-4,6,8-triethyl-2,4,9 dodecatrienoate(5),methyl(2Z,6R,8S)-3,6-epoxy-4,6,8-triethyldodeca-2,4-dienoate(6),methyl(2Z,6R,8S)-4,6-diethyl-3,6-epoxy-8-methye(7)。结论:化合物1~7均为首次从该种海绵中分离得到。
Objective: To study the chemical constituents of Plakortis simplex in the South China Sea. Methods: The simple and flat sponge P. simplex in the South China Sea was purified by normal-phase silica gel column chromatography, reversed phase silica gel column chromatography (ODS), gel column chromatography (Sephadex LH-20) and high performance liquid chromatography The chemical constituents were isolated and purified. The structures of the compounds were identified by physical and chemical properties, spectral analysis methods and literature references. Results: Seven compounds were isolated and identified from the methanol extract of this sponge: (22E) -5α, 8α-epidioxyergosta-6,22-dien-3β-ol (3) monotriajaponide A (4), methyl (2Z, 6R, 8R, 9E) -3,6-epoxy-4,6,8-triethyl-2,4,9 dodecatrienoate (2Z, 6R, 8S) -3,6-epoxy-4,6,8-triethyldodeca-2,4-dienoate epoxy-8-methye (7). Conclusion: Compounds 1 ~ 7 were isolated from this sponge for the first time.