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目的 建立促癌剂检测模型。方法 采用电穿孔的方法 ,将突变的ｃ Ｈａ ｒａｓＶ1 2Ｇ 基因转入人胚肺成纤维细胞ＷＩ 38及人支气管上皮细胞ＢＥＡＳ 2Ｂ中 ,将获得的Ｇ418抗性(Ｇ418ｒ)单克隆细胞株进行ＤＮＡ ＤＯＴ Ｂｌｏｔｉｎｇ及ＲＴ ＰＣＲ分析后 ,进一步用促癌剂佛波?
Objective To establish a cancer promoter assay model. METHODS: The mutated cHa rasV1 2G gene was transferred into human embryonic lung fibroblast WI 38 and human bronchial epithelial cell BEAS 2B by electroporation, and the obtained G418-resistant (G418r) monoclonal cell line was subjected to DNA DOT. After Bloting and RT PCR analysis, further use of cancer-promoting agents Pok Pok?