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目的 研究雾化吸入γ-干扰素对免疫低下大鼠肺部抗感染能力的增强作用。方法 醋酸考的松皮下注射复制大鼠免疫低下模型 ,检测气管内注射白色念珠菌、或气管内注射白色念珠菌并雾化吸入γ-干扰素 1、3、7天的免疫低下大鼠肺泡巨噬细胞的吞噬及杀菌功能 ,抗原提呈功能 ,以及培养上清中 TNF-α活性 ,并于第 7天进行左肺白色念珠菌培养计数。结果 雾化吸入γ-干扰素组大鼠肺白色念珠菌计数显著少于免疫低下组大鼠 (P<0 .0 5 )。雾化吸入γ-干扰素组大鼠肺泡巨噬细胞的吞噬及杀菌百分率显著高于免疫低下组大鼠 (P<0 .0 1)。雾化吸入γ-干扰素组大鼠肺泡巨噬细胞培养上清中 TNF-α的活性显著高于免疫低下组大鼠 (P<0 .0 5 )。结论 雾化吸入γ-干扰素可明显增强免疫低下大鼠肺部抗感染能力
Objective To study the effects of inhaled interferon-gamma on the anti-infective ability of lung in immunocompromised rats. Methods Cortisol was injected subcutaneously in a subcutaneous injection to replicate the immunosuppressive model in rats. The model of Candida albicans was injected intratracheally or Candida albicans was intratracheally instilled and the level of alveolar macrophages Phagocytosis and bactericidal function of the phagocytes, antigen presenting function, and TNF-α activity in the culture supernatant, and C. albicans culture count was counted on the 7th day. Results The count of Candida albicans in the inhaled IFN-γ group was significantly less than that in the immunocompromised group (P <0.05). The percentage of phagocytic and bactericidal percentage of alveolar macrophages in inhaled IFN-γ group was significantly higher than that in immunocompromised group (P <0.01). The activity of TNF-α in the culture supernatant of alveolar macrophages induced by inhaled IFN-γ was significantly higher than that in immunocompromised rats (P <0.05). Conclusion Inhalation of interferon-gamma can significantly enhance the anti-infection ability of lung in immunocompromised rats