采用高效液相色谱法，Ｃ１８柱，以ｐＨ４０的磷酸二氢钾－乙腈（７０∶３０）为流动相，检测波长２１０ｎｍ，测定阿齐霉素的含量。在１６０～２０００μｇ／ｍＬ范围内，浓度与峰面积线性关系良好（ｒ＝０９９９９）。阿齐霉素的平均回收率为１００８％，精密度ＲＳＤ为０９％（ｎ＝６），方法简便、准确 High performance liquid chromatography (HPLC) and C18 column were used to determine the azithromycin content at a wavelength of 210 nm with potassium dihydrogen phosphate - acetonitrile (70:30) at pH4.0 as the mobile phase. In the range of 160-2000 μg / mL, the linear relationship between concentration and peak area was good (r = 0.9999). The mean recoveries of azithromycin were 100.8% and the precision RSD was 0.9% (n = 6). The method was simple and accurate