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为促进短距槽舌兰的资源驯化栽培,本文对其生物学特性、生长发育规律和组织培养快速繁殖技术进行了研究。对开花植株进行人工授粉,取种子进行观察发现,授粉后140 d种子才达到成熟状态;将其接种在无植物生长调节剂的RE培养基上,萌发率可达90%以上。将萌发的种子转接在RE+0.2 mg·L-1 NAA+0.2 mg·L-1 6-BA+5 g·L-1椰粉+2 g·L-1水解酪蛋白的培养基上可诱导原球茎大量增殖。最适壮苗生根培养基为RE+0.6 mg·L-1 NAA+0.2 mg·L-1 6-BA+80 g·L-1香蕉泥,生根率100%。试管苗移栽到直径0.3~1.7 cm的小号细树皮盆栽中,成活率在95%以上,试管苗栽培6个月后可分化花芽,这为短距槽舌兰的产业化生产奠定了基础。
In order to promote domestication and cultivation of short-tangential agave, this paper studied its biological characteristics, growth and development rules and rapid propagation of tissue culture. The flowering plants artificial pollination, the seeds were observed and found that 140 d after pollination to mature seeds; inoculated in the absence of plant growth regulators RE medium, the germination rate of up to 90%. The germinating seeds were transferred on the medium of RE + 0.2 mg · L-1 NAA + 0.2 mg · L-1 6-BA + 5 g · L -1 coconut milk + 2 g · L-1 hydrolyzed casein Induction of protocorm multiplication. The best rooting medium was RE + 0.6 mg · L-1 NAA + 0.2 mg · L-1 6-BA + 80 g · L-1 banana puree, and the rooting rate was 100%. The survival rate of the test tube seedlings transplanted into the small bark pots 0.3 ~ 1.7 cm in diameter was more than 95%, and the flower buds could be differentiated after 6 months’ culture in vitro. This laid the foundation for the industrialization of short - basis.