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目的:通过观察16 Hz/130 dB次声作用大鼠不同时间其血浆超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)水平的变化,揭示次声作用对大鼠氧化应激损伤的特点。方法:80只SD大鼠随机分为次声作用1、7、14、21和28 d组及对应的假暴露组,每组8只大鼠。次声暴露组每日定时在次声舱内接受16 Hz/130 dB次声作用2h,连续1、7、14、21和28 d,假暴露组除不施加次声作用外,其在次声舱中放置的时间、次数、采样时间点均和次声暴露组相同。次声暴露后即刻测定大鼠血浆SOD、MDA、NO水平,测得的结果与假暴露组进行比较。结果:与假暴露组相比,大鼠血浆SOD活力在次声作用后显著降低(P<0.05),且随次声作用时间延长,SOD活力逐渐降低;大鼠血浆MDA含量在次声作用后显著升高(P<0.05),且随次声作用时间延长,MDA含量逐渐升高;大鼠血浆NO含量在次声作用7、14 d时显著降低(P<0.05),1、21和28 d时无统计学差异(P>0.05)。结论:次声作用可引起大鼠氧化应激损伤,损伤的程度与次声暴露时间有关。
OBJECTIVE: To observe the changes of plasma superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO) levels at different times of 16 Hz / 130 dB in rats, Characteristics of oxidative stress injury in rats. Methods: Eighty SD rats were randomly divided into infrasound group 1, 7, 14, 21 and 28 d and corresponding sham exposure group, 8 rats in each group. The infrasound exposed group received daily 16 Hz / 130 dB infrasound for 2 hours in the infrasound for 1, 7, 14, 21 and 28 days. The sham-exposed group, except for infrasound, The time, number of times and sampling time points in the cabin are the same as the infrasound exposure group. Immediately after infrasound, plasma SOD, MDA and NO levels were measured. The measured results were compared with sham-exposed groups. Results: Compared with sham-exposed group, the activity of SOD in plasma decreased significantly after infrasound (P <0.05), and the activity of SOD decreased gradually with infrasound time; the level of plasma MDA in rats decreased significantly after infrasound (P <0.05), and the content of MDA gradually increased with the prolonged infrasound time. The content of NO in plasma decreased significantly (P <0.05) d no statistical difference (P> 0.05). Conclusion: Infrasound can cause oxidative stress injury in rats, the degree of injury is related to infrasound exposure time.