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在观察35例人正常食管上皮及13例食管鳞癌超微结构的基础上,本文利用刀豆球蛋白A-辣根过氧化物酶-3.3二氨基联苯胺-四氧化锇(ConA-HRP-DAB-O_sO_4)方法,对人正常食管及食管鳞癌细胞表面的ConA受体进行光镜和电镜观察。电镜观察结果为人正常上皮的的表浅细胞及棘细胞胞体及胞突的细胞膜上有电子致密物质。胞浆内糖原颗粒、脂滴界膜、内质网池及核膜上亦有电子致密物质。桥粒、张力原纤维、膜包颗粒未见明显的电子致密物质。本文的观察结果与文献上描述的人正常食管上皮的ConA受体分布是一致的。
After observing the ultrastructure of 35 cases of normal esophageal epithelium and 13 cases of esophageal squamous cell carcinoma, we used concanavalin A-horseradish peroxidase-3.3 diaminobenzidine-osmium tetroxide (ConA-HRP- DAB-O_sO_4 method was used to observe the ConA receptor on the surface of normal esophageal and esophageal squamous cell carcinoma cells by light and electron microscopy. The results of electron microscopy showed that there were electron dense substances on the superficial cells of human normal epithelium and the cell membranes of spine cells and cytoplasms. Intracytoplasmic glycogen granules, lipid droplets, endoplasmic reticulum pool, and nuclear membrane also contain electron-dense substances. No significant electron-dense material was observed in the desmosomes, tensile fibrils, and membrane-coated particles. The observations in this article are consistent with the distribution of ConA receptors in normal human esophageal epithelium described in the literature.