目的　建立高效液相法测定脑组织氨基酸类神经递质的方法。方法　利用反相高效液相色谱二元梯度洗脱系统，邻苯二甲醛柱前衍生法结合荧光检测器测定脑组织中６ 种氨基酸类神经递质（天冬氨酸、谷氨酸、谷氨酰胺、γ－ 氨基丁酸、甘氨酸、牛磺酸）的含量。结果　６ 种氨基酸类神经递质在１３ ｍｉｎ 内得到良好分离，在１～１０ μｍｏｌ／Ｌ浓度范围内与峰高的线性关系较好。结论　本方法具有快速、准确、灵敏度高、重现性好等优点。 Objective To establish a HPLC method for the determination of amino acid neurotransmitters in brain tissue. Methods The reversed-phase high performance liquid chromatography binary gradient elution system was used. The determination of six amino acid neurotransmitters (aspartic acid, glutamic acid, glutamine Amide, gamma-aminobutyric acid, glycine, taurine). Results Six kinds of amino acid neurotransmitters were well separated within 13 min. The linear relationship was good with the peak height in the range of 1-10 μmol / L. Conclusion The method has the advantages of fast, accurate, high sensitivity and good reproducibility.