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目的检测职业性氟接触人群血清中可溶性Sema4D(sSema4D)水平,探讨sSema4D与氟负荷的相关性。方法选取湖北省某铝业公司91名氟接触工人和54名行政职员为研究对象。应用问卷法调查其人口统计学特征。采用离子选择电极法测定尿氟。采用酶联免疫吸附试验(ELISA)法检测sSema4D水平。应用SPSS 17.0软件对数据进行统计分析。结果氟接触工人无氟性骨损伤组(APW-FBI)的尿氟(UF)水平(4.83±2.62 mg/L)高于对照组(1.30±0.11 mg/L)(P<0.01),氟接触工人有氟性骨损伤组(APW+FBI)的UF水平(15.0±3.35 mg/L)高于APW-FBI组(P<0.01);APW-FBI组的sSema4D水平(2.99±0.30 ng/mL)低于对照组(5.84±0.06 ng/mL)(P<0.01),APW+FBI组的sSema4D水平(1.90±0.24 ng/mL)低于APW-FBI组(P<0.01);UF与sSema4D呈负相关(r=-0.76,P<0.01)。结论氟接触工人(尤其氟性骨损伤)sSema4D水平较低,且与UF水平呈负相关。
Objective To detect the level of soluble Sema4D (sSema4D) in sera of occupational fluorine-exposed people and to explore the correlation between sSema4D and fluoride load. Methods A total of 91 fluorine-exposed workers and 54 administrative staff from an aluminum company in Hubei Province were selected as research objects. To investigate the demographic characteristics by questionnaire. Determination of urinary fluoride using ion-selective electrode method. The level of sSema4D was detected by enzyme-linked immunosorbent assay (ELISA). SPSS 17.0 software was used to analyze the data. Results Compared with the control group (1.30 ± 0.11 mg / L), the level of urinary fluoride (UF) in fluorine-exposed workers without fluoride-induced bone injury (APW-FBI) was 4.83 ± 2.62 mg / The level of sFma4D (2.99 ± 0.30 ng / mL) in APW-FBI group was significantly higher than that in APW-FBI group (15.0 ± 3.35 mg / L) The level of sSema4D in APW + FBI group (1.90 ± 0.24 ng / mL) was lower than that in APW-FBI group (P <0.01). UF and sSema4D were negative (R = -0.76, P <0.01). Conclusion The level of sSema4D in fluoride exposed workers (especially fluorosis) is low and negatively correlated with the level of UF.