[Objective] To optimize the enrichment technology of atractylenolideⅠ and Ⅲ by macroporous resin in the crude extracts of RHIZOMA ATRACTYLODIS MACROCEPHALAE. [Methods]Enrichment effects of six macroporous resins on atractylenolideⅠ and Ⅲ were compared by static adsorption and desorption. Macroporous resin having good research results were selected for the dynamic test. [Results] The optimal technology parameters of atractylenolide enrichment by HP20 resin were as follows: concentrations of atractylenolideⅠ and Ⅲ were 25. 6and 59. 7 μg / mL,respectively; sampling quantity was 4 BV and flow rate was 2 BV / h. Impurities were removed by 16 BV water and 6 BV of30% ethanol in turn. Then,elution was carried out by 12 BV of 75% ethanol at 2 BV / h velocity. Under these conditions,the recovery rates of atractylenolideⅠ and Ⅲ were 100% and 98. 24%,respectively. [Conclusions]Enrichment of atractylenolideⅠ and Ⅲ by HP20 macroporou resin was simple and feasible. [Objective] To optimize the enrichment technology of atractylenolide I and III by macroporous resins in the crude extracts of RHIZOMA ATRACTYLODIS MACROCEPHALAE. [Methods] Enrichment effects of six macroporous resins on atractylenolide I and III were compared by static adsorption and desorption. Macroporous resin having good research Results were selected for the dynamic test. [Results] The optimal technology parameters of atractylenolide enrichment by HP20 resin were as follows: concentrations of atractylenolide I and III were 25. 6 and 59. 7 μg / mL, respectively; sampling quantity was 4 BV and flow rate was 2 BV / h. Impurities were removed by 16 BV water and 6 BV of 30% ethanol in turn. Then, elution was carried out by 12 BV of 75% ethanol at 2 BV / h velocity. Under these conditions, the recovery rates of atractylenolide I and III were 100% and 98.24%, respectively. [Conclusions] Enrichment of atractylenolide I and III by HP20 macropropylene resin was simple and feasible.