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目的研究精浆弹性硬蛋白酶、白细胞介素(IL)-6以及IL-23在慢性生殖道炎症患者精液中的表达水平及其与精液参数的关系。方法收集男性不育患者精液标本60例,健康生育男性精液标本20例作对照组。每份标本按照世界卫生组织《人类精液检验与处理实验室手册》第五版标准进行分析处理,同时行白细胞CD45染色、精子染色质结构完整性实验以及采用酶联免疫吸附实验(ELISA)方法检测精浆弹性硬蛋白酶、IL-6和IL-23表达水平。结果精浆IL-6、IL-23与精子总数、存活率、活力具有显著负相关性,并且IL-6与精子DNA碎片化指数呈显著正相关性(r=0.645,P<0.01)。精浆IL-6、IL-23在两组之间差异具有统计学意义,且二者之间呈正相关(r=0.625,P<0.01)。精浆弹性硬蛋白酶表达水平与精液量、精子密度呈负相关性(P均<0.05)。结论精浆炎症因子弹性硬蛋白酶、IL-6和IL-23对诊断慢性生殖道炎症具有提示意义,并且精浆IL-6和IL-23可能对精液质量影响更大。
Objective To study the expression of seminal plasma elastase, interleukin (IL) -6 and IL-23 in the seminal fluid of patients with chronic genital tract inflammation and its relationship with semen parameters. Methods Sixty male semen samples were collected from male infertility patients and 20 healthy male reproductive sperm samples were used as control group. Each specimen was analyzed and processed according to the fifth edition of the WHO Manual on Human Semen Test and Processing, meanwhile CD45 staining of leukocytes, structural integrity of chromatin of sperm and enzyme-linked immunosorbent assay (ELISA) were performed Seminal elastase, IL-6 and IL-23 expression levels. Results The seminal plasma IL-6 and IL-23 had a significant negative correlation with the total number of sperm, survival rate and vitality. There was a significant positive correlation between IL-6 and sperm DNA fragmentation index (r = 0.645, P <0.01). There was a significant difference between the two groups in the seminal plasma IL-6 and IL-23 levels (r = 0.625, P <0.01). Seminal plasma elastase expression was negatively correlated with seminal fluid volume and sperm density (P <0.05). Conclusion Seminal plasma elastin elastase, IL-6 and IL-23 may be valuable for the diagnosis of chronic genital tract inflammation. Seminal fluid IL-6 and IL-23 may have a greater impact on semen quality.