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目的从膜脂流动性等观察葛根素对谷氨酸所致大鼠原代神经细胞损伤的保护作用。方法建立大鼠原代神经细胞谷氨酸损伤模型,以1,6-二苯基-1,3,5-己三烯(DPH)为探针,用荧光偏振法测定荧光偏振度(P)值和微粘度(η),研究细胞膜脂流动性的改变;MTT(四甲基偶氮唑盐)法、培养介质乳酸脱氢酶(LDH)活力测定观察葛根素的保护作用。结果葛根素组荧光偏振度和微粘度低于谷氨酸损伤组,与尼莫地平作用相当,且呈现剂量依赖关系。葛根素组原代神经细胞LDH的释放减少,吸光度增加,但葛根素对正常细胞增殖无影响。结论葛根素对谷氨酸所致的大鼠原代神经细胞损伤具有保护作用,其保护作用可能与葛根素改善神经细胞膜脂流动性有关。
Objective To observe the protective effect of puerarin on glutamate-induced primary neuronal injury in rats from the perspective of membrane fluidity. Methods The glutamate injury model of rat primary neural cells was established. The fluorescence polarization degree was measured by fluorescence polarization method using 1,6-diphenyl-1,3,5-hexatriene (DPH) as a probe. The value and microviscosity (η) were used to study the change of cell membrane lipid fluidity; the protective effect of puerarin was observed by MTT (methylnitrazine salt) method and culture medium lactate dehydrogenase (LDH) activity assay. Results The fluorescence polarization degree and microviscosity of puerarin group were lower than that of glutamate injury group, which was equivalent to that of nimodipine and showed a dose-dependent relationship. The release of LDH from primary neurons in the puerarin group was decreased and the absorbance was increased, but puerarin had no effect on normal cell proliferation. Conclusion Puerarin has a protective effect on glutamate-induced primary neuronal damage in rats, and its protective effect may be related to the improvement of plasma membrane lipid fluidity of puerarin.