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目的:建立同时测定糙苏中3个苯乙醇苷含量的HPLC法。方法:采用Agilent Zorbax SB C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈-磷酸二氢钾缓冲盐(pH=2.5)20∶80等度洗脱,流速1.0 mL·min-1,检测波长334 nm。结果:连翘酯苷B在0.32～1.60μg,类叶升麻苷在0.48～2.40μg,异类叶升麻苷在0.36～1.80μg与峰面积呈良好的线性关系;r分别为0.9999,0.9995,0.9998。平均加样回收率(n=3)分别为99.6%,99.4%,98.6%;RSD分别为0.88%,0.40%,1.0%。结论:本方法快速简便、灵敏、可靠,为土家族民间药物糙苏的质量评价提供了科学依据。
Objective: To establish a HPLC method for the simultaneous determination of 3 phenylethanoid glycosides in Rough. METHODS: Agilent Zorbax SB C18 column (4.6 mm × 250 mm, 5 μm) was used as mobile phase. The mobile phase consisted of isocratic elution of acetonitrile-potassium dihydrogen phosphate buffer (pH = 2.5) at 20:80 with a flow rate of 1.0 mL · min -1 , Detection wavelength 334 nm. Results: Forsythoside B was 0.32-1.60μg, the content of acteoside was 0.48-2.40μg, and the content of isoacteoside was 0.36-1.80μg, with a good linear relationship with the peak area; r was 0.9999, 0.9999, 0.9998. The average recoveries (n = 3) were 99.6%, 99.4% and 98.6%, respectively. The RSDs were 0.88%, 0.40% and 1.0%, respectively. Conclusion: The method is rapid, simple, sensitive and reliable, which provides a scientific basis for the quality evaluation of Tujia folk medicine.