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分别将酮洛芬与牛血清白蛋白(BSA)及卵清蛋白(OVA)偶联制得免疫原和包被原,经过免疫新西兰白兔制备多克隆抗体,抗体经纯化后效价为1:128000。使用自制的抗体,建立了测定酮洛芬的间接竞争酶联免疫吸附(ic-ELISA)新方法。ic-ELISA的线性范围为0.010～10.0μg/L,IC50为0.235μg/L,最低检测限为0.0040μg/L,线性回归方程为y=-22.97ρ+104.5(R2=0.980),与布洛芬、双氯酚酸的交叉反应率均小于4%,方法可用于水体中酮洛芬的检测。
Ketoprofen was conjugated with bovine serum albumin (BSA) and ovalbumin (OVA) respectively to obtain the immunogen and the original coating. The polyclonal antibody was prepared from the immunized New Zealand white rabbits. The titer of the purified antibody was 1: 128000. A new method of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established for the determination of ketoprofen using self-made antibodies. The linear range of ic-ELISA was 0.010 ~ 10.0μg / L, IC50 was 0.235μg / L, the lowest detection limit was 0.0040μg / L, the linear regression equation was y = -22.97ρ + 104.5 (R2 = 0.980) Fen, diclofenac acid cross-reactivity were less than 4%, the method can be used for the determination of ketoprofen in water.