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目的观察清热化瘀滋阴方的抗炎镇痛作用和对狼疮性MRL/lpr小鼠肾组织凋亡蛋白的影响。方法制备小鼠醋酸扭体和二甲苯致小鼠耳肿胀模型,动物分为5组,模型组、3个剂量(12,24,48 g·kg-1)清热化瘀滋阴方组及阿司匹林组(300 mg·kg-1),灌胃3 d,观察给药前后镇痛抗炎作用。制备大鼠Ⅲ型变态反应模型,动物分为5组,模型组、3个剂量(6,12,24 g·kg-1)清热化瘀滋阴方及泼尼松组(5 mg·kg-1),灌胃10 d,观察给药后大鼠皮肤反应程度。MRL/lpr小鼠分为4组,模型组、清热化瘀滋阴方组(24 g·kg-1)、泼尼松组(10mg·kg-1)及清热化瘀滋阴方联合泼尼松组(24 g·kg-1+10 mg·kg-1),灌胃4周,检测对肾组织Fas、Fas L凋亡蛋白的表达。结果清热化瘀滋阴方能够明显减少扭体次数和耳廓肿胀度;抑制大鼠Ⅲ型变态反应皮肤反应程度;同时显著升高MRL/lpr小鼠肾组织Fas、Fas L蛋白表达。结论清热化瘀滋阴方具有抗炎镇痛、抑制变态反应和促进肾组织细胞凋亡作用。
Objective To observe the anti-inflammatory and analgesic effects of Qingre Huayu Ziyin Recipe and its effect on the apoptotic protein in renal tissue of lupus-borne MRL / lpr mice. Methods Mouse ear swelling model induced by acetic acid writhing and xylene was established. The animals were divided into 5 groups: model group, Qingre Huayu Zijinfang group (3 doses) and aspirin (12,24,48 g · kg-1) (300 mg · kg-1) for 3 days. The analgesic and anti-inflammatory effects were observed before and after administration. The rats were divided into 5 groups: model group, 3 groups of 6,12,24 g · kg-1 Qingre Huayu recipe and prednisone group (5 mg · kg- 1), gavage for 10 days to observe the degree of skin reaction in rats after administration. The MRL / lpr mice were divided into 4 groups: model group, Qingre Huayu Ziyin Recipe (24 g · kg -1), prednisone group (10 mg · kg -1) and Qingre Huayu Ziyin Recipe (24 g · kg-1 and 10 mg · kg-1) for 4 weeks. The expressions of Fas and Fas L-apoptotic protein in kidney were measured. Results Qingre Huayu Recipe can significantly reduce the number of writhing and auricle swelling degree; inhibit the level of allergic reaction in rats with type Ⅲ allergy; at the same time, significantly increase the expression of Fas and Fas L protein in the kidney of MRL / lpr mice. Conclusion Qingre Huayu Recipe has anti-inflammatory and analgesic effects, inhibits allergic reaction and promotes apoptosis of renal tissue.