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目的 了解大鼠内淋巴囊的细胞增殖状态。方法 选用 10只健康成年S D大鼠 ,腹腔注射溴脱氧尿嘧啶核苷 (BrdU)后 ,取颞骨作组织学处理 ,用抗BrdU单克隆抗体 ,通过免疫组织化学染色 ,观察内淋巴囊组织中增殖细胞的定位与分布。结果 大鼠内淋巴囊中间部的上皮、上皮下区域和囊腔内存在着散在的BrdU阳性细胞 ,而在耳蜗未发现阳性细胞。结论 大鼠内淋巴囊具有细胞增殖能力 ,可能是内耳具有细胞更新功能的独特部位。
Objective To understand the cell proliferation status of endolymphatic sac in rats. Methods Ten healthy adult SD rats were injected intraperitoneally with bromodeoxyuridine (BrdU). The temporal bone was taken for histological treatment. Anti-BrdU monoclonal antibody was used to detect the proliferation of endolymphatic sac tissue by immunohistochemical staining Cell location and distribution. Results There were scattered BrdU positive cells in the epithelium, subepithelial region and cystic cavity in the middle part of the lymphatic sac in rats, but no positive cells were found in the cochlea. Conclusion The endolymphatic sac in rats has the ability of cell proliferation, which may be a unique site of cellular renewal in the inner ear.