Genetic Transformation of Rice with Pi-d2 Gene Enhances Resistance to Rice Blast Fungus Magnaporthe

来源 :Rice Science | 被引量 : 0次 | 上传用户:free522
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
The gene Pi-d2,conferring gene-for-gene resistance to the Chinese blast strain ZB15,was isolated from a rice variety(Digu) by the map-based cloning strategy.Here,we constructed a control plasmid pZH01-pi-d2tp309(pZH01-tp309) and three different expression constructs,pCB-Pi-d25.3kb(pCB5.3kb),pCB-Pi-d26.3kb(pCB6.3kb) and pZH01-Pi-d22.72kb(pZH01-2.72kb) of Pi-d2,driven by Pi-d2 gene’s own promoter or CaMV35S promoter.These constructs were separately introduced into japonica rice varieties Lijiangxintuanhegu,Taipei 309,Nipponbare and Zhonghua 9 through Agrobacterium-mediated transformation.A total of 150 transgenic rice plants were obtained from the regenerated calli selected on hygromycin.PCR,RT-PCR and Southern-blotting assay showed that the gene of interest had been integrated into rice genome and stably inherited.Thirty-five transgenic lines independently derived from T1 progeny were inoculated with the rice blast strain ZB15.Transformants exhibited resistance to rice blast at various levels.The lesions on the transgenic plant leaves were less severe than those on the controls and the resistance level of transgenic plants harboring the gene of interest from three vectors had no difference.The own promoter of Pi-d2,about 2.2 kb or 3.2 kb,had the similar promoter function as CaMV35S.Field evaluation for three successive years supported the results of artificial trial,and some lines with high resistance to rice leaf blast and neck blast were obtained. The gene Pi-d2, conferring gene-for-gene resistance to the Chinese blast strain ZB15, was isolated from a rice variety (Digu) by the map-based cloning strategy. Here, we constructed a control plasmid pZH01-pi-d2tp309 pZH01-tp309) and three different expression constructs pCB-Pi-d25.3kb (pCB5.3kb), pCB-Pi-d26.3kb (pCB6.3kb) and pZH01-Pi-d22.72kb (pZH01-2.72kb) of Pi-d2, driven by Pi-d2 gene’s own promoter or CaMV35S promoter.These constructs were all introduced into japonica rice varieties Lijiangxintuanhegu, Taipei 309, Nipponbare and Zhonghua 9 through Agrobacterium-mediated transformation. A total of 150 transgenic rice plants were obtained from the regenerated calli selected on hygromycin. PCR, RT-PCR and Southern-blotting assay showed that the gene of interest had been integrated into rice genome and stably inherited. Thirty-five transgenic lines independently derived from T1 progeny were inoculated with the rice blast strain ZB15.Transformants phenol resistance to rice blast at various levels.The lesions on the transgenic plant leaves were less severe than those on the controls and the resistance level of transgenic plants harboring the gene of interest from three vectors had no difference. The proprietary promoter of Pi-d2, about 2.2 kb or 3.2 kb , had the similar promoter function as CaMV35S.Field evaluation for three successive years supported the results of artificial trial, and some lines with high resistance to rice leaf blast and neck blast were obtained.
其他文献
由于现代经济社会快速发展的需要,我国对电力资源的需求量也在不断增大.作为国家重要的基础设施建设之一——供电企业,其所发挥的作用越来越明显.对于供电企业来说,保证用电
本文通过对荣华二采区10
连日来,洞庭湖高洪压境,湖南省湘阴县堤垸险情不断.按照县防汛指挥部的部署,白马镇水电站站长李某负责白马镇机埠堤段的防守工作.但据岳阳市、湘阴县纪委调查,自7月3日以来,
本文通过对荣华二采区10
从紫砂壶诞生的明代开始,紫砂装饰就已出现。紫砂壶“花货”从壶身到壶嘴的多部位皆有采用仿生原理制作的;“光货”与“筋囊器”则是通过立体线条对紫砂进行装饰,此点现今也
阐述混合现实技术的特点及与医学的密切关系,综述混合现实技术在医学教育、精准手术和医患沟通等方面的具体应用,以期为今后开展基于混合现实技术的医学探索研究提供参考依据
期刊
随着我国各项事业的不断发展,对建材质量也提出了更高的要求,各种材料的应用,使建材品种增多,建筑物对建材的选择也越来越丰富,这也就给建材的质量检验提出了全新标准.市场经
目的探讨腹腔镜子宫肌瘤剔除术与传统开腹手术治疗子宫肌瘤患者的临床效果比较。方法选取2014年3月至2015年3月沈阳市妇婴医院收治的58例子宫肌瘤患者作为研究对象,按治疗方