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AIM:To study the expression of cyclooxygenase-2(COX-2)gene in gastric cancer and the relationship between COX-2expression and clinicopathologic features of gastric cancer.METHODS:With reference to the expression of β-actin gene,COX-2 mRNA level was examined in cancerous tissues andadjacent noncancerous mucosa from 33 patients bysemiquantitative reverse transcription-polymerase chainreaction(RT-PCR).Quantitation of relative band Adj volumecounts was performed using molecular Analyst for windowssoftware.The COX-2 index was determined from the band Adjvolume counts ratio of COX-2 to constitutively expressed actin.RESULTS:The COX-2 index in gastric carcinoma wassignificantly higher than that in normal mucosa(0.5966±0.2659vs 0.2979±0.171,u=5.4309,P<0.01).Significantly higherexpression of COX-2 mRNA was also observed in patientswith lymph node involvement than that in those without(0.6775±0.2486 vs 0.4105±0.2182,t=2.9341,P<0.01).Furthermore,the staging in the UICC TNM classificationsignificantly correlated with COX-2 overexpression(F=3.656,P<0.05),the COX-2 index in stage Ⅲ and Ⅳ was significantlyhigher than those in stage Ⅰ and Ⅱ(q=3.2728 and q=3.4906,P<0.05).The COX-2 index showed no correlationwith patient’s age,sex,blood group,tumor location,grosstyping,depth of invasion,differentiation,and the greatesttumor dimension(P>0.05).CONCLUSION:Expression of COX-2 mRNA in gastriccarcinoma was significantly higher,which may enhancelymphatic metastasis in patients with gastric carcinoma.Thestaging in the UICC TNM classification was significantlycorrelated with COX-2 over-expression.COX-2 may contributeto progression of tumor in human gastric adenocarcinoma.
AIM: To study the expression of cyclooxygenase-2 (COX-2) gene in gastric cancer and the relationship between COX-2 expression and clinicopathologic features of gastric cancer. METHODS: With reference to the expression of β-actin gene, COX- 2 mRNA level was examined in cancerous tissues andadjacent noncancerous mucosa from 33 patients by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). Quantitation of relative band Adj volume counts was performed using molecular Analyst for window software of. COX-2 index was determined from the band Adjvolume counts ratio of COX-2 to constitutively expressed actin .RESULTS: The COX-2 index in gastric carcinoma wassignificantly higher than that in normal mucosa (0.5966 ± 0.2659 vs. 0.2979 ± 0.171, u = 5.4309, P <0.01) .Significantly higherexpression of COX- 2 mRNA was also observed in patients with lymph node involvement than that in those without (0.6775 ± 0.2486 vs 0.4105 ± 0.2182, t = 2.9341, P <0.01) .Furthermore, the staging in the UICC TNM classificationsign The COX-2 index in stage III and IV was significantly higher than those in stage I and II (q = 3.2728 and q = 3.4906, P <0.05). The COX-2 index showed no correlation with patient’s age, sex, blood group, tumor location, grosstyping, depth of invasion, differentiation, and the greatest tumor dimension (P> 0.05) .CONCLUSION: Expression of COX-2 mRNA in gastriccarcinoma was significantly higher , which may enhancelymphatic metastasis in patients with gastric carcinoma. Ticking in the UICC TNM classification was significantlycorrelated with COX-2 over-expression. COX-2 may contributing to progression of tumor in human gastric adenocarcinoma.