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本文介绍用辣根过氧化物酶(HRP)标记葡萄球菌A蛋白(SPA),以人胰为抗原组织,滴加待测血清,再加HRP-SPA及显色剂,检测糖尿病(DM)患者血清胰岛细胞浆抗体(ICA)。本法有下列优点:(1)方法简便,不需特殊设备,检测结果可长期保存;(2)采用Bouin液固定石腊包埋切片,抗原保存性好,便于随访患者ICA效价追踪:(3)非特异性染色少,不受血型物质干扰。用本法检测19例Ⅰ型DM、83例Ⅱ型DM及102例正常人,ICA阳性率分别为5例(26.32％)、4例(4.82％)及2例(1.96％)。Ⅰ型DM与Ⅱ型DM及正常者阳性率之间均有显著差异(P<
This article describes the use of horseradish peroxidase (HRP) labeled staphylococcal protein A (SPA), human pancreatic antigen organization, dropping the serum to be measured, coupled with HRP-SPA and color reagent, detection of diabetes (DM) patients Serum islet cytoplasmic antibody (ICA). This method has the following advantages: (1) The method is simple, without special equipment, the test results can be stored for a long time; (2) using Bouin fixed paraffin embedded sections, antigen preservation is good, easy follow-up ICA patient tracking: 3) non-specific staining less blood type substances. Nineteen cases of type I DM, 83 cases of type II DM and 102 normal controls were detected by this method. The positive rates of ICA were 5 cases (26.32%), 4 cases (4.82%) and 2 cases (1.96%) respectively. There was a significant difference between the positive rate of type I DM and normal type II DM (P <