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目的 利用组织工程技术构建自体成骨细胞 -生物陶瓷复合体并应用于大鼠颅骨极量骨缺损模型的修复。 方法 30 0~ 35 0g雄性SD大鼠 2 0只随机分为细胞 -陶瓷复合体组和单纯生物陶瓷对照组。所有大鼠均在全麻及无菌条件下取其左侧股骨及胫骨 ,将骨髓冲入 75cm2培养瓶中 ,在诱导培养条件下进行体外扩增分化 ,其中实验组细胞在培养 14d后消化接种于经纤维连接蛋白表面修饰的含孔磷酸钙陶瓷上继续培养 15d ,再将细胞 -陶瓷复合体用于与细胞来源对应的大鼠颅骨极量缺损 (直径 8mm)的修复 ;而对照组仅植入经表面修饰的无细胞陶瓷。每隔 4周为 1个时相点分别处死每组 2只大鼠直至 2 0周 ,从形态学及组织学角度观察比较颅骨缺损修复愈合情况。 结果 无论X线摄片及组织切片均表明 ,实验组颅骨极量缺损修复优于同期对照组。 结论 以含孔磷酸钙陶瓷作为支架的自体成骨细胞移植具有明显的骨缺损修复能力。
OBJECTIVE: To construct an autologous osteoblast-bioceramic composite by tissue engineering technique and apply it to the repair of maximal bone defect in rat skull. Methods Twenty male Sprague-Dawley rats weighing 30 ~ 35 g were randomly divided into a cell-ceramic composite group and a bioceramic control group. All rats under general anesthesia and aseptic conditions to take the left femur and tibia, the bone marrow into the 75cm2 flask, in vitro culture under the conditions of expansion and differentiation, in which the experimental group after 14 days of culture digestion On the fibronectin-surface modified porous calcium phosphate ceramics, the cells were cultured for 15 days, then the cell-ceramic complexes were used to repair the maximal skull defects (diameter 8mm) in rat skull cells corresponding to the cell sources. Surface-modified cell-free ceramic. Two rabbits in each group were sacrificed at intervals of 1 every 4 weeks until 20 weeks. Morphology and histology were used to compare the healing and repair of skull defects. Results Both X-ray and histological sections showed that the skull bone defect repair in the experimental group was better than that in the control group. Conclusion Autologous osteoblast transplantation using porous calcium phosphate ceramics as a scaffold has obvious capability of repairing bone defects.