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作者提出用高压液相色谱法(HPLC)测定牛乳和干酪中的V_A,平均回收率为93±6%,重复性的相对标准差为±4%,检测极限为20ng松香油(皂化后的V_A)。作者将此法所得结果与常规的比色法作了对比,二者在统计学上无显著差异。比色法每日最多能测6份且需使用相当大量的实验器材,HPLC法则每日可测30份样品,所需玻璃器皿可减少1/3。此法简单、快速,特异性强、重复性好。方法 [试剂]石油醚(40～60℃),连苯三酚,无水硫酸钠和氢氧化钾(以上均为Merck分析级试剂),HPLC用乙腈(USA)。
The authors proposed high pressure liquid chromatography (HPLC) determination of V_A in milk and cheese with an average recovery of 93 ± 6%, repeatability of ± 4% relative standard deviation and a limit of detection of 20 ng of rosin oil (saponified V_A ). The authors compared the results obtained with this method with the conventional colorimetric method, with no significant difference between the two. The colorimetric method can measure up to 6 samples per day and uses a considerable amount of experimental equipment. The HPLC method can measure up to 30 samples per day and the required glassware can be reduced by 1/3. This method is simple, fast, specific, reproducible. Method [Reagents] Petroleum ether (40-60 ° C), pyrogallol, anhydrous sodium sulfate and potassium hydroxide (all Merck analytical grade reagents above), acetonitrile for HPLC (USA).