The swine effluent studied was collected from scale pig farms,located in Yujiang County of Jiangxi Province,China,and duckweed(Spriodela polyrrhiza) was selected to dispose the effluent.The purpose of this study was to elucidate the effects of duckweed growth on the dissolved organic matter composition in swine effluent.Throughout the experiment period,the concentrations of organic matter were determined regularly,and the excitationemission matrix(3DEEM) spectroscopy was used to characterize the fluorescence component.Compared with no-duckweed treatments(controls),the specific ultra-violet absorbance at 254 nm(SUVA_(254)) was increased by a final average of 34.4%as the phytoremediation using duckweed,and the removal rate of DOC was increased by a final average of 28.0%.In swine effluent,four fluorescence components were identified,including two protein-like(tryptophan,tyrosine) and two humic-like(fulvic acids,humic acids) components.For all treatments,the concentrations of protein-like components decreased by a final average of 69.0%.As the growth of duckweed,the concentrations of humic-like components were increased by a final average of 123.5%than controls.Significant and positive correlations were observed between SUVA_(254) and humic-like components.Compared with the controls,the humification index(HIX) increased by a final average of 9.0%for duckweed treatments.Meanwhile,the duckweed growth leaded to a lower biological index(BIX) and a higher proportion of microbial-derived fulvic acids than controls.In conclusion,the duckweed remediation not only enhanced the removal rate of organic matter in swine effluent,but also increased the percent of humic substances. The swine effluent was was collected from scale pig farms, located in Yujiang County of Jiangxi Province, China, and duckweed (Spriodela polyrrhiza) was selected to dispose the effluent. The purpose of this study was to elucidate the effects of duckweed growth on the dissolved organic matter composition in swine effluent. Throughout the experiment period, the concentrations of organic matter were determined regularly, and the excitationemission matrix (3DEEM) spectroscopy was used to characterize the fluorescence component. Compared with no-duckweed treatments (controls), the specific ultra -UV absorbance at 254 nm (SUVA_ (254)) was increased by a final average of 34.4% as the phytoremediation using duckweed, and the removal rate of DOC was increased by a final average of 28.0%. In swine effluent, four fluorescence components were identified, including two protein-like (tryptophan, tyrosine) and two humic-like (fulvic acids, humic acids) components. For all treatments, the concentrations of protein-like components decreased by a final average of 69.0% .As the growth of duckweed, the concentrations of humic-like components were increased by a final average of 123.5% than controls. Identification and positive correlations were observed between SUVA 254 and humic-like components. Compared with the controls, the humification index (HIX) increased by a final average of 9.0% for duckweed treatments. Meanwhile while the duckweed growth leaded to a lower biological index (BIX) and a higher proportion of microbial-derived fulvic acids than controls.In conclusion, the duckweed remediation not only enhanced the removal rate of organic matter in swine effluent, but also increased the percentage of humic substances.