论文部分内容阅读
目的:观察肉桂醛对高脂饮食C57BL/6小鼠骨代谢的影响,探讨其与组织蛋白酶K(Cathepsin K)表达的相关性。方法:肉桂醛治疗高脂喂养的C57BL/6小鼠8周后,分别取小鼠的股骨和胫骨,用HE染色和茜素红染色观察骨微结构变化、骨代谢以及骨发育情况;用质构仪评价骨生物力学性能;用免疫组织化学法观察Cathepsin K在骨组织中的表达情况。结果:肉桂醛治疗8周后,高脂饮食小鼠骨小梁断裂程度减轻,排列整齐,骨形成明显增多(P<0.05);骨的硬度形变量、第一循环硬度和峰值压力均明显升高。同时,肉桂醛能明显降低骨组织Cathepsin K蛋白表达(P<0.05)。结论:肉桂醛能改善高脂饮食喂养的C57BL/6小鼠骨强度,改善骨微结构,改善骨代谢,其作用机制可能是与调节Cathepsin K的表达有关。
Objective: To observe the effect of cinnamaldehyde on bone metabolism in high-fat diet C57BL / 6 mice and to explore its relationship with the expression of cathepsin K (Cathepsin K). Methods: After cinnamic aldehyde treatment for 8 weeks in C57BL / 6 mice fed with high fat diet, the femur and tibia of mice were taken respectively. The changes of microarchitecture, bone metabolism and bone development were observed by HE staining and alizarin red staining. The structure and instrument were used to evaluate the biomechanical properties of bone. The expression of Cathepsin K in bone was observed by immunohistochemistry. RESULTS: After cinnamic aldehyde treatment for 8 weeks, the fracture of trabecular bone in mice fed with high-fat diet was alleviated and neatly arranged, and the bone formation was significantly increased (P <0.05). The hardness, the first cycle hardness and the peak pressure of bone were significantly increased high. At the same time, cinnamaldehyde significantly reduced Cathepsin K protein expression in bone tissue (P <0.05). CONCLUSION: Cinnamaldehyde can improve the bone strength, improve the micro-structure and improve the bone metabolism in C57BL / 6 mice fed with a high-fat diet. The mechanism may be related to the regulation of Cathepsin K expression.