论文部分内容阅读
本文将正常值精液分为两组,每组10例。A组为液氮冷冻精液;B组为新鲜精液,作为对照组。在A、B两组之间分别进行光镜和电镜下精子对比观察。结果;1.光镜下两组精子畸形率基本一致。2.电镜下,A组精子的形态、生物膜及亚细胞结构基本完整;与B组对照,A组的主要改变局限于精子头部的顶体和中段的线粒体,顶体变化为其前段肿胀、分离和弯曲,有的部位仅保留一层顶体内膜,线粒体则呈基质密度降低、结构模糊,少数可见线粒体空泡。本文还围绕超低温保存对精子超微结构的影响以及该影响对精子运动和授精功能的作用进行了讨论。
In this paper, normal semen will be divided into two groups, 10 cases in each group. Group A is liquid nitrogen frozen semen; Group B is fresh semen as control group. In A, B between the two groups were observed under light microscope and electron microscope sperm contrast. Results; 1. Under the light microscope two groups of sperm deformity rate basically the same. Under electron microscope, the morphology, biofilm and subcellular structure of group A were basically intact. Compared with group B, the main changes of group A were confined to the acrosome and middle mitochondria in sperm head, , Separation and bending, and some sites only retained a layer of acrosomal intima, mitochondria showed a decrease in matrix density, fuzzy structure, a few visible mitochondrial vacuoles. This article also discussed the effect of cryopreservation on the ultrastructure of sperm and the effect of the effect on sperm motility and insemination function.