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目的:研究冬凌草甲素(oridonin,ORI)诱导人食管癌SHEE细胞凋亡的效应及其机制。方法:用不同浓度ORI分别作用人食管癌SHEE细胞株不同时间,噻唑蓝比色法(MTT)检测细胞生长抑制率;透射电镜观察细胞超微结构及改变;原位末端标记/碘化吡啶(TUNEL/PI)双染流式细胞术检测细胞凋亡率和细胞周期。结果:ORI对SHEE细胞的生长有显著的抑制作用,64μg/ml的ORI作用24h、48h及72h对SHEE细胞生长的抑制率分别为78.7%、89.5%及91.7%;32μg/ml的ORI作用2h后,电镜下可见线粒体在细胞核出现变化之前已发生形状改变,作用8h后,线粒体内部结构消失,细胞核呈细胞凋亡的典型改变;作用24h后,流式细胞仪检测凋亡率为47.7%,细胞周期图像显示明显的凋亡峰。结论:ORI可诱导SHEE细胞凋亡,其机制可能与线粒体凋亡途径有关。
Objective: To investigate the effect and mechanism of oridonin (ORI) on apoptosis in human esophageal SHEE cells. Methods: The human esophageal SHEE cells were treated with different concentrations of ORI for different times. MTT assay was used to detect the cell growth inhibition rate. Transmission electron microscopy was used to observe the ultrastructure and change of SHEE cells. In situ terminal labeling / TUNEL / PI) double staining flow cytometry was used to detect the apoptosis rate and cell cycle. ORI inhibited the growth of SHEE cells at 64μg / ml for 24 h, 48 h and 72 h, respectively. The inhibitory rates of ORI on SHEE cells were 78.7%, 89.5% and 91.7%, respectively. ORI of 32 μg / ml for 2 h After electron microscopy, mitochondria had changed shape before the change of nucleus. After 8h, the internal structure of mitochondria disappeared and the nucleus showed a typical change of apoptosis. After 24h, the apoptosis rate was 47.7% by flow cytometry, Cell cycle images show significant apoptotic peaks. Conclusion: ORI can induce apoptosis in SHEE cells, which may be related to mitochondrial apoptosis pathway.