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目的 探索放射辐照对异体神经移植组织学改变的影响。方法 将 18只SD大白鼠随机均分为三组 :自体对照组、异体对照组、辐照处理组。应用放射辐照方法处理异体神经后进行移植。术后 12周移植神经和胫前肌HE染色 ,移植神经S - 10 0蛋白及波形蛋白免疫组化和甲苯胺蓝染色 ,观察其组织学变化和计数单位面积内阳性轴突数和再生的有髓神经纤维数量。结果 放射辐照处理后的移植神经 ,纤维组织增生和炎性细胞浸润较少 ,胫前肌生长良好 ,再生有髓神经纤维增多 (与异体对照组比较 ,P <0 .0 5 ) ,单位面积内的阳性轴突数明显增多 (P <0 .0 5 )。结论 放射辐照处理可降低异体神经的抗原性
Objective To explore the effect of radiation on the histological changes of allogeneic nerve grafts. Methods Eighteen SD rats were randomly divided into three groups: autologous control group, allograft control group and irradiation group. Application of radiation irradiation after allogeneic nerve transplantation. At 12 weeks after operation, the transplanted nerves and tibialis anterior muscle were stained with HE, the S - 10 protein of the transplanted nerve and vimentin immunohistochemistry and toluidine blue staining were used to observe the histological changes and count the number of positive axons per unit area and regeneration The number of myelinated fibers. Results After irradiation, there were fewer grafts and infiltrating inflammatory cells in the transplanted nerve. The anterior tibialis muscle grew well and the number of regenerated myelinated nerve fibers increased (compared with the allograft control group, P <0.05), and the unit area Within the positive axon number was significantly increased (P <0. 05). Conclusion Radiation irradiation can reduce the antigenicity of allogeneic nerve