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目的:通过定性研究探讨人胎肝细胞在体外与丙型肝炎病毒(HCV)阳性血清共培养24h是否能感染HCV。方法:人胎肝细胞在体外与HCV阳性血清共培养24h后,采用原位RT-PCR技术对其进行HCV定性检测。结果:通过原位RT-PCR技术检测,发现人胎肝细胞内HCV正、负链RNA均为阳性。此外,将人胎肝细胞在体外与HCV阳性血清共培养24h并经多次洗涤后继续培养的上清液加入到新鲜分离的正常人胎肝细胞培养基中培养,可以得到上述相同的鲒果。结论:人胎肝细胞在体外培养情况下可以感染HCV,并支持HCV在其内复制,产生具有传染性的HCV颗粒分泌到培养液中。
OBJECTIVE: To investigate whether human fetal liver cells can be infected with HCV in vitro after co-cultured with hepatitis C virus (HCV) -positive sera for 24 hours. Methods: Human fetal hepatocytes were co-cultured with HCV-positive sera in vitro for 24 hours and then detected by using in situ RT-PCR. Results: Positive and negative HCV RNA was positive in human fetal hepatocytes by in situ RT-PCR. In addition, human fetal hepatocytes were cultured in vitro with HCV-positive sera for 24 h and the supernatants after multiple washes were added to freshly isolated normal human fetal hepatocyte medium to obtain the same fruits as above . CONCLUSION: Human fetal hepatocytes can infect HCV in vitro and support the replication of HCV in it, resulting in the secretion of infectious HCV particles into the culture medium.